Harley Quinn (also referred to as Harley Quinn: The Animated Series) is an animated adult-theme comedy series. Created by Patrick Schumacker, Justine Halpern and Dean Lorey, the series centers on Harley Quinn as she attempts to make a name for herself in Gotham City's criminal landscape, with the help her roommate Poison Ivy and other villains in the DC universe.
The first two season were released on the DC Universe streaming service, while the third is expected to released on HBO Max. The first two seasons subsequently moved to the latter after the former was "restructured" in September 2020.
Harley Quinn who has finally broken things off once and for all with the Joker and attempts to make it on her own as the criminal Queenpin of Gotham City. The series features Harley Quinn, Poison Ivy and a whole cast of heroes and villains, old and new, from the DC Universe.
- Kaley Cuoco as Harleen Quinzel / Harley Quinn: The main protagonist who is the runt of the chain gang in Arkham Asylum at the very beginning of the series of the same name. However, as the series progresses, she recently escapes custody with her cellmate, Ivy, and sets off on a most epic adventure into becoming criminal Queenpin of Gotham City.
- Lake Bell as Pamela Isley / Poison Ivy: Sarcastic and incredibly smart, Ivy is Harley’s best friend and now roommate. Very much a loner (she prefers plants over people), Ivy is sometimes the voice of reason but always a true friend to Harley. Ivy soon becomes her reluctant partner-in-crime.
- Alan Tudyk as:
- The Joker: Equal parts homicidal and charismatic, he’s been Harley’s longtime awful boyfriend, but when Harley breaks up with him, she vows to take his cherished number-one spot on the Most Wanted list. The Joker thinks of Harley as his creation and, as she grows in power, does everything he can to prevent it from happening;
- Basil Karlo / Clayface: A large, humanoid mass of clay, Clayface was a struggling, trained actor until a terrible pottery accident turned him into the shapeshifting villain he is now. His aspirations for stardom and his ability to transform his appearance to look like anyone should help him land some roles but he’s such a terrible actor that the only work he can get is with Harley’s crew, pretending to be whoever they need him to be help with a heist. He still holds out hope that he’ll land one of the many auditions he fruitlessly goes on. Jonah Platt provides Clayface's singing voice
- Julian Day / Calendar Man
- Condiment King
- Jason Alexander as Sy Borgman: Once upon a time, Sy used to be a suave, super spy. Now in his 90s, he’s a wheelchair user with most of his body replaced by creaky 50s era bionic parts. Crotchety, difficult and longs to become relevant again. He’s also Harley and Ivy’s landlord, and insists they take him on their heists so can get back into the action – or else he won’t repair the plumbing.
- J. B. Smoove as Frank the Plant: A sentient plant owned by Poison Ivy.
- Ron Funches as King Shark: His lower half is human, but his upper half is the body of a great white shark. Even though he looks incredibly fierce, he’s a gentle soul who just wants to be normal. That is, until he smells blood. And then he goes into a frenzy and literally eats everyone around him leaving death and carnage everywhere. Then feels bad about it.
- Tony Hale as Dr. Psycho: An angry misogynist dwarf with telekinesis. He was a major villain until the “incident” ten years ago when in the midst of losing a battle against Wonder Woman he became frustrated and publicly called her 'the C-word'. Even other villains were disgusted. Disgraced, the only one who’ll have him is Harley. A woman. He can’t stand it, but he’ll do anything to get back into the villain community.
- Diedrich Bader as Bruce Wayne / Batman: A fully deputized special agent to the Gotham Police Department who turned Harley in and brought her to the Arkham Asylum for a life sentence behind bars prior to this series. His alter-ego, Bruce Wayne, is a director to a community service program which enforces Arkham's inmates under hard labor until they are allowed to return to society.
- Matt Oberg as
- Charles Brown / Kite Man: A kite-themed criminal and Poison Ivy's initial love interest.
- Waylon Jones / Killer Croc
- James Adomian as
- Bane: A venom-addicted criminal who loves setting off explosives.
- Andy Dale as Harvey Dent / Two-Face
- Giancarlo Esposito as Lex Luthor: The leader of the Legion of Doom
- Alfred Molina as Mr. Freeze
- Tom Hollander as Alfred Pennyworth: Batman's butler and surrogate father.
- Wanda Sykes as Tsaritsa / Queen of Fables
- Natalie Morales as Lois Lane
- Jim Rash as Edward Nigma / The Riddler
- Christopher Meloni as Commissioner James Gordon
- Rahul Kohli as Jonathan Crane / Scarecrow
- Sanna Lathan as Selina Kyle / Catwoman
- Vanessa Marshall as Princess Diana / Wonder Woman
- Jacob Tremblay as Damian Wayne / Robin: The boy wonder and Batman's sidekick who helped turn Harley Quinn in prior to the series. At the beginning of the first episode, Harley was ordered by Batman to stand on a platform scale with height meter so that he can measure her weight and height for a correct sized prison uniform.
- Tisha Campbell-Martin as Tawny Young and MONICA
- Phil LaMarr as David Hyde / Black Manta
- Briana Cuoco as Barbara Gordon / Batgirl
- Wayne Knight as Oswald Cobblepot / The Penguin
- Sean Giamborne as Joshua Cobblepot
- Charlie Adler as Nick Quinzel, Grandpa Quinzel
- Chris Diamantopoulos as Arthur Curry / Aquaman
- Susie Essman as Sharon Quinzel, Grandma Quinzel
- Meryl Hathaway as Marcus
- Jameela Jamil as Eris
- Tom Kenny as Clayface's Hand
- George Lopez as Himself
- Justina Machado as Bethany
- Howie Mandel as Himself
- Natalie Morales as Lois Lane
- Brad Morris as Victor Zsasz
- Frankie Muniz as Himself
- Suzy Nakamura as Realtor
- Rhea Perlman as Golda
- Scott Porter as The Flash
- Nicole Sullivan as Mrs. Cobblepot, Benjamin
- Talia Tabin as Debbie Day
- Jessica Walter as Granny Goodness, Wendy Brown
- Mark Whitten as Herman Cizko / The Cowled Critic
In June 2021, DC announced a tie-in comic book limited series titled Harley Quinn: The Animated Series - The Eat, Bang, Kill Tour. The series will focus on Harley and Ivy after the events of Season 2 and bridge the gap to the third season. The series is expected for release in September 2021.