- "It's time to go green!"
- ―Poison Ivy[src]
Upon escaping from Arkham Asylum, Poison Ivy teams up with the Riddler, Mr. Freeze, Two-Face and Clayface to rob Gotham Bullion Fortress by stealing a key, a freeze-ray, instantly-growing-tree-seeds, and a laser-cannon.
Poison Ivy helps the Riddler and Two-Face escape after Batman and Robin destroy the Two-Face's armored-truck during a chase. Afterwards, Poison Ivy goes to Botanical Gardens with the Riddler and assists him in stealing instant-grow tree seeds. Whereas the Riddler escapes and leaves a clue for the Dynamic Duo once again, Poison Ivy is defeated by Batman and Robin.
Poison Ivy is last seen in her cell at Arkham Asylum, watering plants.
Poison Ivy is one of the villains who The Joker breaks out of Arkham, and attempts to escape with Bane and The Penguin on Bane's Mole Machine. However, Robin blocks their path with ice, and when Bane tries to tunnel out, he comes out in Arkham's confines and impales the Mole Machine high in Arkham's walls, thwarting their efforts to escape. Furious with his failure, Poison Ivy hits Bane on the head repeatedly with Penguin's umbrella. However, the villains cause an even larger breakout shortly afterward.
Poison Ivy proceeds to the Gotham Botanic Gardens. After she is tracked down, she can be defeated and bought for use in Free Play.
- In the Nintendo D.S. version of the videogame, Poison Ivy can walk tightropes, use acrobatic poles, and is immune to toxins.
- In the console-versions of the videogame, Poison Ivy can double-jump and has an immunity to toxins. She can also blow toxins at opponents and perform her signature kiss of death.
- In LEGO Batman 2, Poison Ivy retains her double-jump and toxin immunity, and gains a vine-whip and the ability to perform a backflip. She can also travel between special patches of flowers.